Induction of bacterial luciferase by pure oxygen
نویسندگان
چکیده
منابع مشابه
Structure of bacterial luciferase.
The generation of light by living organisms such as fireflies, glow-worms, mushrooms, fish, or bacteria growing on decaying materials has been a subject of fascination throughout the ages, partly because it occurs without the need for high temperatures. The chemistry behind the numerous bioluminescent systems is quite varied, and the enzymes that catalyze the reactions, the luciferases, are a l...
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Irradiation of bacterial luciferase results in a long lived luminescence which is qualitatively similar to the chemically induced bioluminescence. It has been found that oxygen is required for the emission of photoexcited bioluminescence, but that it need not be present during irradiation. Oxygen must be added promptly, however, for the intermediate has a relatively short lifetime. In the absen...
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By using FMN enriched in (13)C (90%) at position C-4a, we have conclusively shown that the reaction of molecular oxygen with bacterial luciferase-bound FMNH(2) forms an adduct at the 4a position. Consistent with this are (13)C NMR studies of FMN and other flavin compounds which show that this carbon should be unusually reactive in the reduced 1,5-dihydroflavins with respect to electrophilic att...
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ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1992
ISSN: 0022-1287
DOI: 10.1099/00221287-138-4-831